Coding

Part:BBa_K3360004:Design

Designed by: Li Wenxi   Group: iGEM20_SZU-China   (2020-10-15)
Revision as of 21:25, 23 October 2020 by Hamlet (Talk | contribs) (→‎Source)


endo-beta-1_4-glucanase from Streptomyces


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 431
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Encoding the endo-beta-1,4-glucanase of Streptomyces,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.

Source

Encoding the endo-beta-1,4-glucanase of Streptomyces,the entire sequence has been optimized by E.coli codons, and the N-terminus is marked with GST to increase its solubility and reduce the formation of inclusion bodies.

References