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Coding
Part:BBa_K3692002
Designed by: Juli Mukhadze Group: iGEM20_Estonia_TUIT (2020-10-07)
PLIN3
Usage and Biology
PLIN3 is a gene that encodes perilipin - a protein which is involved both in generation of lipid droplets (LDs) and as well as covering of phospholipid monolayer of LDs.
PLIN3 is a human perilipin gene. However, it has been shown that the expression of this gene can increase yields of TAG by 28% in Sacharomyces cerevisiae (Teixeira et al., 2018). A potential reason for this can be that it stabilizes TAGs by improving LD assembly and the budding process.
References
- Teixeira, P. G., David, F., Siewers, V., & Nielsen, J. (2018). Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae. FEMS Yeast Research, 18(6). https://doi.org/10.1093/femsyr/foy060
Sequence and Features
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal SpeI site found at 369
Illegal PstI site found at 218 - 12INCOMPATIBLE WITH RFC[12]Illegal SpeI site found at 369
Illegal PstI site found at 218 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal SpeI site found at 369
Illegal PstI site found at 218 - 25INCOMPATIBLE WITH RFC[25]Illegal SpeI site found at 369
Illegal PstI site found at 218 - 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |