Composite
Part:BBa_K3606079:Design
Designed by: Yuanyuan Liu Group: iGEM20_Fudan (2020-10-23)
P1 driven mcbABCD; PtetR driven mcbEFG; luxPR driven tetR
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5559
Illegal XbaI site found at 5544
Illegal XbaI site found at 6868
Illegal SpeI site found at 5972
Illegal PstI site found at 2382
Illegal PstI site found at 2415
Illegal PstI site found at 5958 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5559
Illegal NheI site found at 6930
Illegal SpeI site found at 5972
Illegal PstI site found at 2382
Illegal PstI site found at 2415
Illegal PstI site found at 5958
Illegal NotI site found at 5551
Illegal NotI site found at 5963 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5559
Illegal BamHI site found at 3378
Illegal XhoI site found at 5709 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5559
Illegal XbaI site found at 5544
Illegal XbaI site found at 6868
Illegal SpeI site found at 5972
Illegal PstI site found at 2382
Illegal PstI site found at 2415
Illegal PstI site found at 5958 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5559
Illegal XbaI site found at 5544
Illegal XbaI site found at 6868
Illegal SpeI site found at 5972
Illegal PstI site found at 2382
Illegal PstI site found at 2415
Illegal PstI site found at 5958
Illegal NgoMIV site found at 2044
Illegal AgeI site found at 2216 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
tba
Source
tba