Coding
Part:BBa_K3407008
Designed by: Maartje Spaans Group: iGEM20_TUDelft (2020-10-23)
sgRNA targetting 4.3 gene of T7 bacteriophage
The sgRNA encoded on this part targets a protospacer in gene 4.3 of the wildtype T7 phage. Therefore, it can be used together with a plasmid encoding Cas9 to select T7 phages engineered in the position of 4.3 gene, as the wild type T7 phage genome will be cleaved.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
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Categories
Parameters
None |