Coding

Part:BBa_K3407008

Designed by: Maartje Spaans   Group: iGEM20_TUDelft   (2020-10-23)
Revision as of 19:57, 23 October 2020 by Registry (Talk | contribs)

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sgRNA targetting 4.3 gene of T7 bacteriophage

The sgRNA encoded on this part targets a protospacer in gene 4.3 of the wildtype T7 phage. Therefore, it can be used together with a plasmid encoding Cas9 to select T7 phages engineered in the position of 4.3 gene, as the wild type T7 phage genome will be cleaved.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None