Composite

Part:BBa_K3606067:Design

Designed by: Yuanyuan Liu   Group: iGEM20_Fudan   (2020-10-23)
Revision as of 19:42, 23 October 2020 by Registry (Talk | contribs)

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P11 driven mcbABCD and PtetR driven mcbEFG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 5548
    Illegal XbaI site found at 5533
    Illegal PstI site found at 2371
    Illegal PstI site found at 2404
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 5548
    Illegal PstI site found at 2371
    Illegal PstI site found at 2404
    Illegal NotI site found at 5540
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 5548
    Illegal BamHI site found at 3367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 5548
    Illegal XbaI site found at 5533
    Illegal PstI site found at 2371
    Illegal PstI site found at 2404
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 5548
    Illegal XbaI site found at 5533
    Illegal PstI site found at 2371
    Illegal PstI site found at 2404
    Illegal NgoMIV site found at 2033
    Illegal AgeI site found at 2205
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

tba


Source

tba

References