Composite
Part:BBa_K3606066:Design
Designed by: Yuanyuan Liu Group: iGEM20_Fudan (2020-10-23)
P10 driven mcbABCD and PtetR driven mcbEFG
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5548
Illegal XbaI site found at 5533
Illegal PstI site found at 2371
Illegal PstI site found at 2404 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5548
Illegal PstI site found at 2371
Illegal PstI site found at 2404
Illegal NotI site found at 5540 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5548
Illegal BamHI site found at 3367 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5548
Illegal XbaI site found at 5533
Illegal PstI site found at 2371
Illegal PstI site found at 2404 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5548
Illegal XbaI site found at 5533
Illegal PstI site found at 2371
Illegal PstI site found at 2404
Illegal NgoMIV site found at 2033
Illegal AgeI site found at 2205 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
tba
Source
tba