Composite
Part:BBa_K3606065:Design
Designed by: Yuanyuan Liu Group: iGEM20_Fudan (2020-10-23)
P9 driven mcbABCD and PtetR driven mcbEFG
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal PstI site found at 2370
Illegal PstI site found at 2403 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 5547
Illegal PstI site found at 2370
Illegal PstI site found at 2403
Illegal NotI site found at 5539 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 5547
Illegal BamHI site found at 3366 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal PstI site found at 2370
Illegal PstI site found at 2403 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 5547
Illegal XbaI site found at 5532
Illegal PstI site found at 2370
Illegal PstI site found at 2403
Illegal NgoMIV site found at 2032
Illegal AgeI site found at 2204 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
tba
Source
tba