RNA

Part:BBa_K3380501:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-09)
Revision as of 18:29, 23 October 2020 by Alexandru popov (Talk | contribs) (→‎Source)


Broccoli fluorescent RNA aptamer construct under T7 RNA polymerase promoter (BBa_z0251)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


Design Notes

This construct has not been tested in the lab, however the Edinburgh iGEM 2020 team tested the BBa_K3380500 construct. The BBa_K3380501 construct can be assembled following a similar protocol (on the experience page of the BBA_K3380500 construct).

Source

The parts were acquired as de novo synthesized sequences from Sigma-Aldrich.

References

Millacura, F.A., Li, M., Valenzuela-Ortega, M.A. and French, C., 2019. TXO: Transcription-Only genetic circuits as a novel cell-free approach for Synthetic Biology. bioRxiv, p.826230.