RNA

Part:BBa_K3380501:Design

Designed by: Alexandru Popov   Group: iGEM20_Edinburgh   (2020-10-09)
Revision as of 18:27, 23 October 2020 by Alexandru popov (Talk | contribs) (→‎Design Notes)


Broccoli fluorescent RNA aptamer construct under T7 RNA polymerase promoter (BBa_z0251)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 25


Design Notes

This construct has not been tested in the lab, however the Edinburgh iGEM 2020 team tested the BBa_K3380500 construct. The BBa_K3380501 construct can be assembled following a similar protocol (on the experience page of the BBA_K3380500 construct).

Source

Acquired as a sequence (de novo synthesized).

References