Coding

Part:BBa_K3692002

Designed by: Juli Mukhadze   Group: iGEM20_Estonia_TUIT   (2020-10-07)
Revision as of 18:26, 23 October 2020 by Lytvynen (Talk | contribs)


PLIN3


PLIN3 is a gene that encodes perilipin - a protein which is involved both in generation of lipid droplets (LDs) and as well as covering of phospholipid monolayer of LDs.

PLIN3 is a human perilipin gene. However, it has been shown that the expression of this gene can increase yields of TAG by 28% in Sacharomyces cerevisiae (Teixeira et al., 2018). A potential reason for this can be that it stabilizes TAGs by improving LD assembly and the budding process.

References

  • Teixeira, P. G., David, F., Siewers, V., & Nielsen, J. (2018). Engineering lipid droplet assembly mechanisms for improved triacylglycerol accumulation in Saccharomyces cerevisiae. FEMS Yeast Research, 18(6). https://doi.org/10.1093/femsyr/foy060

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 369
    Illegal PstI site found at 218
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 369
    Illegal PstI site found at 218
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 369
    Illegal PstI site found at 218
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 369
    Illegal PstI site found at 218
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None