Coding

Part:BBa_K3582026:Design

Designed by: Saillesh Aravindhan C   Group: iGEM20_IISER-Pune-India   (2020-10-20)
Revision as of 18:12, 23 October 2020 by Sailleshac (Talk | contribs) (→‎References)

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Inhibitory sequence 1 of PfEMP1 - ICAM1 interaction grafted into a cyclotide


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 112
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 143
    Illegal BsaI.rc site found at 109


Design Notes

  • 1] RFC10 criteria satisfied
  • 2] Strep Ⅱ tag gene inserted in loop 3
  • 3] Codon optimized with E-coli K-12 strain
  • 4] Inhibitor 1 grafted in loop 6
  • 5] C and N intein added for circularization



Source

Reverse translation of amino acid sequence

References