Composite
Part:BBa_K3431051
Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang Group: iGEM20_CSMU_Taiwan (2020-10-18)
Banana_ToeholdSwitch-Regulated Invertase
The is an invertase (BBa_K3431000) generating device regulated by Banana Toehold Switch (BBa_K3431039), with T7 Promoter (BBa_I719005) and T7 Terminator (BBa_K731721) as recognition sites for T7 RNA Polymerase in PURExpress in vitro protein synthesis kit.
Experiment result
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1441
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1212
Illegal BamHI site found at 1342
Illegal XhoI site found at 1413 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 1013
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |