Composite

Part:BBa_K3431051

Designed by: Jian-An Pan, Cheng-Yang Ma, Yi-Ching Chen, Shen-Lin Chen, Huan-Jui Chang   Group: iGEM20_CSMU_Taiwan   (2020-10-18)
Revision as of 17:52, 23 October 2020 by IrisRChen (Talk | contribs)


Banana_ToeholdSwitch-Regulated Invertase

The is an invertase (BBa_K3431000) generating device regulated by Banana Toehold Switch (BBa_K3431039), with T7 Promoter (BBa_I719005) and T7 Terminator (BBa_K731721) as recognition sites for T7 RNA Polymerase in PURExpress in vitro protein synthesis kit.

Experiment result


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1441
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1212
    Illegal BamHI site found at 1342
    Illegal XhoI site found at 1413
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1013
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None