Composite

Part:BBa_K3440020

Designed by: Victoria Muliadi, Julie Cordier   Group: iGEM20_Stockholm   (2020-10-23)
Revision as of 16:03, 23 October 2020 by Registry (Talk | contribs)

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Oscillation circuit under GFP reporter

Pconst(BBa_J23100)-RBS(BBa_B0034)-RhlR(BBa_C0071)-DT(BBa_B0015)-Prhl(BBa_R0071)-RBS(BBa_B0034)-RhlI(BBa_C0070)-RBS(BBa_B0034)-AiiA(BBa_C0060)-RBS(BBa_B0034)-GFP(BBa_E0040)-DT(BBa_B0015)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1733
    Illegal BamHI site found at 301
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1864
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 776
    Illegal BsaI.rc site found at 3249


[edit]
Categories
//chassis/prokaryote/ecoli
//classic/signalling/receiver
//function/biosynthesis/ahl
//function/degradation/ahl
//function/reporter
//function/reporter/fluorescence
Parameters
originAequorea victoria
outputOscillating GFP production
signalling_moleculeQS of Lux system and Rhl system