Regulatory

Part:BBa_K3338007

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-23)
Revision as of 16:01, 23 October 2020 by Registry (Talk | contribs)

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Synthetic promoter_1 with NF-κB and AP1 binding sites

The presented synthetic promoter is able to recognize LPS in the surrounding of the cell via the Toll-like receptor, NF-κB/AP1 pathway leading to strong induction of gene expression. Due to its relatively low basal activity and strong induction following LPS supplementation it is perfectly suitable for the application as a LPS-sensor. The novel synthetic promoter is based on a minimal CMV-promoter just containing the TATA-box and the Initiator-Sequence of the original CMV-promoter. Upstream of the minimal Promoter three repetitions of randomly generated NF-κB binding sites follwed by randomly generated AP-1 binding sites are localized.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1
    Illegal NotI site found at 58
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//chassis/eukaryote
//chassis/eukaryote/human
//function/sensor
//promoter
//regulation/positive
Parameters
None