Composite

Part:BBa_K3365014:Design

Designed by: JUNYAN LI   Group: iGEM20_SJTU-BioX-Shanghai   (2020-10-23)
Revision as of 15:50, 23 October 2020 by Registry (Talk | contribs)

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pBAD/araC upstream of RFP with inhibition unit containing target


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1913
    Illegal XhoI site found at 1922
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 979
    Illegal AgeI site found at 1789
    Illegal AgeI site found at 1901
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 961


Design Notes

target sequence is part of the PDCD1 gene


Source

transcription inhibition

References