RNA

Part:BBa_K3506050

Designed by: Yiling Chen   Group: iGEM20_BNU-China   (2020-10-23)
Revision as of 14:51, 23 October 2020 by Registry (Talk | contribs)

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gRNA targets ADE2 gene

A small guide RNA (sgRNA) component created by fusing an approximately 20 nt target-sequence RNA (crRNA) to a tailored bacterial tracrRNA. We design the sgRNA targeted on ADE2 to specifically knock out this gene in Cryptococcus neoformans.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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