Composite
Part:BBa_K3490031:Design
Designed by: Ren Hao Tsai Group: iGEM20_NCKU_Tainan (2020-10-23)
The toxin-antitoxin system: hicA-hicB
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 109
Illegal BsaI.rc site found at 149
Design Notes
hicA and hicB need to locate in different copy number plasmid to mediate the expression of hicA and hicB. hicA is located in pSB1C3 with pBad promoter while hicB is located in pSB3K3 with J23100.
Source
Derived from IDT synthesis.