![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3490031
Designed by: Ren Hao Tsai Group: iGEM20_NCKU_Tainan (2020-10-23)
The toxin-antitoxin system: hicA-hicB
The TA system is composed of linked genes, encoding a toxic protein that can inhibit cell growth and an antitoxic protein that neutralizes the toxic protein. HicA is a toxin promoting mRNA degradation, leading to the hibernation of bacteria, while HicB is an antitoxin that can neutralize the effect of HicA. Hence, through regulating the expression of hicA and hicB genes, we can control the growth of bacteria, hibernating the bacteria before the contact lens is used.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 109
Illegal BsaI.rc site found at 149
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Categories
Parameters
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