Composite

Part:BBa_K3490031

Designed by: Ren Hao Tsai   Group: iGEM20_NCKU_Tainan   (2020-10-23)
Revision as of 14:38, 23 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


The toxin-antitoxin system: hicA-hicB

The TA system is composed of linked genes, encoding a toxic protein that can inhibit cell growth and an antitoxic protein that neutralizes the toxic protein. HicA is a toxin promoting mRNA degradation, leading to the hibernation of bacteria, while HicB is an antitoxin that can neutralize the effect of HicA. Hence, through regulating the expression of hicA and hicB genes, we can control the growth of bacteria, hibernating the bacteria before the contact lens is used.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 109
    Illegal BsaI.rc site found at 149


[edit]
Categories
Parameters
None