Part:BBa_K3594014
Cytochrome P450 gene (CYP)
CYP is a large protein complex responsible for the degrading of guaiacol, CYP enters researchers' field of vision mainly as a decomposing product of lignin. Past studies have found that there are several G+ bacteria that can use guaiacol as a carbon source. The first step of their metabolism is mainly catalyzed by cytochrome P450 (CYP), which generates catechol through demethylation. This step of demethylation usually requires some redox chaperone proteins to assist.
In an article from AMB Express in 2019, the authors did a very detailed study on the demethylation process of guaiacol. Their data shows that only two enzymes from the G+ bacteria Rhodococcus rhodochrous need to be prepared, namely Cytochrome P450 (WP_085469912 from R. rhodochrous J3) and Ferredoxin reductase (WP_085469913 from R. rhodochrous J3), and transfer them to G-bacteria Pseudomonas After putida EM42, with or without additional carbon source supplementation, the effective degradation of guaiacol can be achieved.
Therefore, we hope to transfer these two enzymes into Escherichia coli to express and decompose the guaiacol in the intestines of locusts.
References: J. García‑Hidalgo et al., AMB Express. 9 (34) (2019) https://amb-express.springeropen.com/articles/10.1186/s13568-019-0759-8#Tab1
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 315
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 163
Illegal NgoMIV site found at 288
Illegal NgoMIV site found at 763
Illegal NgoMIV site found at 1039
Illegal NgoMIV site found at 1057
Illegal AgeI site found at 399 - 1000COMPATIBLE WITH RFC[1000]
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