Coding

Part:BBa_K3493000:Design

Designed by: Emilie Alexander, Benjamin Ouellet   Group: iGEM20_ULaval   (2020-10-22)
Revision as of 14:29, 23 October 2020 by Angelfcc (Talk | contribs)


G. algens dextranase CDS


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 385
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 385
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 385
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 385
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization for E. coli. Following reports that the Streptococcus mutans homologous dextranase has a higher activity when its N terminal (residues 1-99) and C-terminal (residues 733-850) are deleted (Kim et al. 2011), we removed them from the CDS submitted to the registry.


Source

The protein sequence is available at NCBI (https://www.ncbi.nlm.nih.gov/protein/WP_066432599.1?report=genbank&log$=prottop&blast_rank=700&RID=MZBBN4NZ014). The gene sequence was inferred from the protein sequence using codon optimization for E. coli.

References