Coding

Part:BBa_K3656307

Designed by: Huang feiyu   Group: iGEM20_AHUT-ZJU-China   (2020-10-23)
Revision as of 14:25, 23 October 2020 by Registry (Talk | contribs)

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OT3-Carbonic Anhydrase-Mutant-His

This is an improved part of mutant carbonic anhydrase from Pyrococcus horikoshii OT3 which converts the incoming bicarbonate into carbon dioxide in the carboxysome, a step that is essential for CO2 fixation. We have optimized codon sequence in the coding sequence (CDS) to make the mutant carbonic anhydrase gene more suitable for expression in E. coli. In addition to codon optimization, we have added a His-tag to the C-terminal of the CDS that is used for purification of carbonic anhydrase protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 470
    Illegal XhoI site found at 520
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Parameters
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