![](https://parts.igem.org/images/partbypart/icon_coding.png)
Coding
Part:BBa_K3490000
Designed by: Ren Hao Tsai Group: iGEM20_NCKU_Tainan (2020-10-23)
Nitric oxide synthase [Bacillus subtilis]
During literature research, we found out that Bacillus subtilis carries Nitric Oxide Synthases and has the ability to produce NO. So we cloned Bacillus subtilis' NOS gene under the control of an inducible promoter, pLacI, into a plasmid and transformed it into E. coli. In order to induce our target gene, NOS, with IPTG, we put a LacI and T7 promoter in front of NOS and LacO after it. We chose WM3064 as our bacteria strain because it contains T7 RNA polymerase.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal SapI.rc site found at 151
Illegal SapI.rc site found at 469
[edit]
Categories
Parameters
//awards/basic_part/nominee
None |