Coding

Part:BBa_K3490000

Designed by: Ren Hao Tsai   Group: iGEM20_NCKU_Tainan   (2020-10-23)
Revision as of 13:48, 23 October 2020 by Registry (Talk | contribs)

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Nitric oxide synthase [Bacillus subtilis]

During literature research, we found out that Bacillus subtilis carries Nitric Oxide Synthases and has the ability to produce NO. So we cloned Bacillus subtilis' NOS gene under the control of an inducible promoter, pLacI, into a plasmid and transformed it into E. coli. In order to induce our target gene, NOS, with IPTG, we put a LacI and T7 promoter in front of NOS and LacO after it. We chose WM3064 as our bacteria strain because it contains T7 RNA polymerase.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 151
    Illegal SapI.rc site found at 469


[edit]
Categories
//awards/basic_part/nominee
Parameters
None