Regulatory

Part:BBa_K3425104

Designed by: Rubén Crespo Blanco   Group: iGEM20_UofUppsala   (2020-10-23)
Revision as of 13:39, 23 October 2020 by Registry (Talk | contribs)

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DBD-CaFF construct (pSB3C11-J23100)

Device expressing BBa_K3425100 in backbone pSB3C11 (BBa_K3425001) with promoter J23100 and B0034.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 2713
    Illegal NheI site found at 1399
    Illegal NheI site found at 2744
    Illegal NheI site found at 2767
    Illegal PstI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 2713
    Illegal BglII site found at 2854
    Illegal BglII site found at 3728
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 2713
    Illegal PstI site found at 12
    Illegal AgeI site found at 990
    Illegal AgeI site found at 1313
    Illegal AgeI site found at 3420
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 2719
    Illegal BsaI.rc site found at 6


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Categories
Parameters
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