Coding

Part:BBa_K3468020:Design

Designed by: Yufei Zhou   Group: iGEM20_TJUSLS_China   (2020-10-19)
Revision as of 13:18, 23 October 2020 by Chloe8 (Talk | contribs)

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PETase N246I


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

By observing and comparing the structure of PETase, appropriate mutations were constructed and the forces between the residues were modified. By finding suitable sites around L249 and I250 for mutations, the hydrophobic interaction force between the residues was enhanced and the hydrophobic core was created, so N246I was designed.

Source

Ideonalla sakaiensis

References