Composite

Part:BBa_K3598042

Designed by: Ruijuan Xiang   Group: iGEM20_Beijing_4ELEVEN   (2020-10-23)
Revision as of 12:24, 23 October 2020 by Registry (Talk | contribs)

(diff) ← Older revision | Latest revision (diff) | Newer revision → (diff)


EilR_EilR promoter_PJExD promoter_sfGFP

This part is a tightly repressible induction system, PJExD promoter is regulated by EilR, and several cationic dyes act as efficient low-cost inducers. sfGFP is used for quantitative analysis of the system.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 916
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 346
    Illegal AgeI site found at 496
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 954


[edit]
Categories
Parameters
None