Part:BBa_K3338006
EGFP
EGFP is a constitutively fluorescent (enhanced) green fluorescent protein derived from Aequorea Victoria first published by Cormack et al. in 1996 with an excitation wavelength of 488 nm and an emission peak at 507 nm. Due to the introduction of a set of point mutations including a mutation effecting folding efficiency under cell culture conditions, it shows a much higher extinction coefficient and brightness than the natural variant of the protein (Cormack et al. 1996, McRae et al. 2005).
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
References
Cormack, B. P., Valdivia, R. H., & Falkow, S. (1996). FACS-optimized mutants of the green fluorescent protein (GFP). Gene, 173(1 Spec No), 33–38.
McRae, S. R., Brown, C. L., & Bushell, G. R. (2005). Rapid purification of EGFP, EYFP, and ECFP with high yield and purity. Protein expression and purification, 41(1), 121–127.
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