Composite
Part:BBa_K3560004
Designed by: ShiDi Xiao Group: iGEM20_XHD-Wuhan-China (2020-10-23)
PGroES-DrRBS-gcd
The gcd gene of Escherichia coli expresses glucose dehydrogenase (GDH), GDH is a membrane-bound quinone protein that catalyzes the conversion of glucose to gluconic acid, generates hydrogen protons, and decrease the pH value. but it needs to be combined with Pyrroloquinoline quinone (PQQ) coenzyme, then the holoenzyme can function normally. Deinococcus radiodurans constitutively expresses PQQ, which can form holoenzymes with GDH from E. coli to catalyze the production of gluconic acid.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 190
Illegal AgeI site found at 1797 - 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |