Composite

Part:BBa_K3560004

Designed by: ShiDi Xiao   Group: iGEM20_XHD-Wuhan-China   (2020-10-23)
Revision as of 09:13, 23 October 2020 by Registry (Talk | contribs)

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PGroES-DrRBS-gcd

The gcd gene of Escherichia coli expresses glucose dehydrogenase (GDH), GDH is a membrane-bound quinone protein that catalyzes the conversion of glucose to gluconic acid, generates hydrogen protons, and decrease the pH value. but it needs to be combined with Pyrroloquinoline quinone (PQQ) coenzyme, then the holoenzyme can function normally. Deinococcus radiodurans constitutively expresses PQQ, which can form holoenzymes with GDH from E. coli to catalyze the production of gluconic acid.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 190
    Illegal AgeI site found at 1797
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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