Part:BBa_K511101

EYFP-FF4x4 MammoBlock

This part is an engineered variant of the original green fluorescent protein from Aequorea victoria that fluoresces yellow. Additionally, this protein has four binding sites for the FF4 micro RNA (miRNA). In the presence of FF4 miRNA, translation of this protein is markedly attenuated.

Figures 1 and 2 show typical levels of EYFP-FF4x4-mediated fluorescence following transfection of HEK-293 cells with EYFP-FF4x4 driven by the Hef1a-LacOid low-level constitutive promoter.

For more information, consult the MIT iGEM 2011 wiki here.

FF4 Binding site Characterization by AFCM-EGYPT 2020

Team:AFCM-Egypt 2020characterized this part by structural modelling and simulation of interaction between ff4 and its binding site using ff4 as an input for a NOR gate that could protect our replicon vaccine vectors from being attacked by innate immunity inside dendritic cells and act as a safety switch inside dendritic cells and act as a safety switch all of which is presented in the following part BBa_K3504019

Figure 2.This figure shows the sbol format of the composite part BBa_K3504023 which acts as an off switch as well as shows the way in which a nor gate works.

Figure 1.This figure illustrate the interaction between FF4 and its binding site that showed energy equal to -3.14kcal\mol.

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 205
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 205
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 205
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 205
  
• 1000
  COMPATIBLE WITH RFC[1000]