![](https://parts.igem.org/images/partbypart/icon_composite.png)
Composite
Part:BBa_K3598021
Designed by: Ruijuan Xiang Group: iGEM20_Beijing_4ELEVEN (2020-10-23)
LacI_LacI promoter_T7 promoter_lac operator_RBS lib_sfGFP_T7 terminator
This part is an inducible expression system. The T7 promoter is regulated by lacI repressor through lacO operator to control the expression of sfGFP. When there is no inducer, sfGFP is not expressed and when the inducer IPTG is added, sfGFP is expressed and the fluorescence intensity increases. We construct a RBS library, this system is used for the construction and quantitative determination of our RBS libraty.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 2038
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 1280
Illegal BsaI.rc site found at 1270
Illegal SapI.rc site found at 1303
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Categories
Parameters
None |