Composite

Part:BBa_K3504023

Designed by: Ahmed Wael   Group: iGEM20_AFCM-Egypt   (2020-10-23)
Revision as of 08:24, 23 October 2020 by Ahmed Wael (Talk | contribs) (→‎Characterization)


Off Switch

Part Description

A composite of parts (BBa_I712004,BBa_K3504019,BBa_K3504008) Which form as a whole an off switch using mir-126 & FF4 Mirna (BBa_K3504012) as as an inputs for a NOR gate that could protect our replicon vaccine vectors from being attacked by innate immunity inside dendritic cells and act as a safety switch

Usage

Characterization

We characterized this part by structural modelling and simulation of interaction between ff4 and its binding site using ff4 as an input for a NOR gate that could protect our replicon vaccine vectors from being attacked by innate immunity inside dendritic cells and act as a safety switch

Figure 2.This figure shows the sbol format of this composite part as well as way in which a nor gate works.
Figure 1.These figures illustrate the RNA Fold structure of multiple MiRNAs comparing there stabilities by free energy thermodynamic and showing there ability to induce down regulation of the circuit and work as an off switch.
Figure 3.This figure illustrate the interaction between FF4 and its binding site that showed energy equal to -3.14kcal\mol.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None