DNA

Part:BBa_K3573000:Design

Designed by: Jungwoo Choe   Group: iGEM20_Korea_HS   (2020-10-10)
Revision as of 03:53, 23 October 2020 by Jchoe21 (Talk | contribs) (→‎Design Notes)


Single chain variable fragment (scFV) recognizing RAS


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This year's project is a continuation of 2019's project. Last year our team (Korea_HS) designed and tested CPP-scFv(P5) for its ability to enter the cell and recognize its cognate target protein, lysozyme in a reducing environment.

In 2020, we have designed hyperstable, cell-penetrating scFv targeting RAS protein (scFv(RAS)) based on the structure of RAS: anti-RAS antibody (PDB ID: 2vh5)

Source

This DNA was synthesized

References