Composite

Part:BBa_K3338010

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-22)
Revision as of 21:38, 22 October 2020 by Registry (Talk | contribs)

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IL-6 P-MagA-P2A-hGLuc (Inflammatory Toxin Sensor)

IL-6 P-MagA-P2A-hGLuc is an inflammatory toxin sensor comprising the IL6-Promoter downstream from the MagA-P2A-hGLuc cassette. This part combines the LPS-sensitivity of the IL6-Promoter with the simultaneous expression of the reporter genes MagA for MRI-detection and Gaussia Luciferase for bioluminescence detection in blood or urine. This construct displays the first step in generating a functional inflammatory toxin sensor for clinical use.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 369
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 369
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 369
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI site found at 2803
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696
    Illegal BsaI.rc site found at 2135
    Illegal BsaI.rc site found at 2684
    Illegal SapI site found at 1825


[edit]
Categories
//chassis/eukaryote/human
//function/reporter
//function/sensor
//regulation/positive
Parameters
None