Regulatory

Part:BBa_K3338005:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-22)
Revision as of 21:02, 22 October 2020 by Jonas Scholz (Talk | contribs) (→‎Design Notes)


Interleukin-6 Promoter mutagenized (IL-6 Pmut)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1347
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 220
    Illegal BamHI site found at 395
    Illegal XhoI site found at 1581
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 340
    Illegal BsaI.rc site found at 387
    Illegal BsaI.rc site found at 696


Design Notes

The original sequence of the IL-6 P was modified so that it is compatible with the RFC10 assembly standard. Therefore, a point mutation in a XbaI site was introduced. There is no predicted clash with a NF-κB or an AP1 transcription factor binding site.

Source

The sequence was synthesized by IDT.

References