Composite
Part:BBa_K3697004
Designed by: Christopher Neimeth Group: iGEM20_Stanford (2020-10-22)
Recombination-based Detection System for B. Subtilis (manP)
This system, once incorporated into the B. Subtilis will act as a detection system for a customizable nucleic acid sequence corresponding to the sequence with homology to the "homology arms" of the system. It acts a detection system, as when exposed to the target sequence a recombination will be triggered causing the excision of the negative selection marker that is flanked by the homology arms (more details on the exact negative selection marker and homology arms used in this sequence can be found in the documentation for parts BBa_K3697002 and BBa_K3697003 respectively).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 679
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 1272
Illegal AgeI site found at 922
Illegal AgeI site found at 1016
Illegal AgeI site found at 2682 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI.rc site found at 822
Illegal SapI site found at 1844
[edit]
Categories
Parameters
//dna
//function/recombination
//function/reporter
//function/recombination
//function/reporter
biology | |
device_type | |
insert |