Composite

Part:BBa_K3697004

Designed by: Christopher Neimeth   Group: iGEM20_Stanford   (2020-10-22)
Revision as of 20:38, 22 October 2020 by Registry (Talk | contribs)

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Recombination-based Detection System for B. Subtilis (manP)

This system, once incorporated into the B. Subtilis will act as a detection system for a customizable nucleic acid sequence corresponding to the sequence with homology to the "homology arms" of the system. It acts a detection system, as when exposed to the target sequence a recombination will be triggered causing the excision of the negative selection marker that is flanked by the homology arms (more details on the exact negative selection marker and homology arms used in this sequence can be found in the documentation for parts BBa_K3697002 and BBa_K3697003 respectively).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 679
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1272
    Illegal AgeI site found at 922
    Illegal AgeI site found at 1016
    Illegal AgeI site found at 2682
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 822
    Illegal SapI site found at 1844


[edit]
Categories
//dna
//function/recombination
//function/reporter
Parameters
biology
device_type
insert