Part:BBa_K3697003

Homology Arms for KanR integration in B. Subtilis

This part is the portion of the pOpen Yeast plasmid between the AarI and MspA1I cut sites. These homology arms (as labelled in the features part of this part page) can be used a couple different ways. One, they could be incorporated into the B. subtilis genome so that any target sequence flanked by these homology arms could be incorporated into the B. subtilis genome. Two, these homology arms could be incorporated into the B. subtilis genome flanking a sequence of interest, then if the target sequence is taken up by B. subtilis (the portion of the pOpen Yeast plasmid between the AarI and MspA1I cut sites) the sequence being flanked by these homology arms could be excised from the B. subtilis genome.

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]