Composite

Part:BBa_K3570001

Designed by: Anton Mykhailiuk   Group: iGEM20_Toulouse_INSA-UPS   (2020-10-22)
Revision as of 19:35, 22 October 2020 by Antonmykhailiuk (Talk | contribs)


Sweet rose taste induction system in S. cerevisiae

Introduction

Design

Fig. 1: Brazzein-CrGES-pUC19. The integrative locus used is yprcΔ15. The selective locus used is LEU2. The gene coding for geraniol synthase (CrGES) comes from C. roseus. The gene coding for brazzein comes from an artificial sequence (LG263246.1). CYC1 and PGK terminators are used. The bidirectional promoter Gal 1/10 is used.


Experiments

Team iGEM Toulouse 2020 did not have sufficient time to complete the cloning and hence, to test this part functionality.

References


Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 4894
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 4894
    Illegal BamHI site found at 3752
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 4894
    Illegal XbaI site found at 3746
    Illegal AgeI site found at 1551
    Illegal AgeI site found at 4507
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None