Regulatory

Part:BBa_K117002:Design

Designed by: Nguyen Xuan Hung   Group: iGEM08_NTU-Singapore   (2008-10-07)
Revision as of 04:56, 29 October 2008 by Dariusaw (Talk | contribs) (→‎Design Notes)

LsrA promoter (indirectly activated by AI-2)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

LsrA is initially commercially synthesized with RBS BBa_B0034 by ATIbiotech in a blue script cloning vector. LsrA is then extracted using PCR with oligo fragments of DNA sequence 5'-GAATTATGTATACATAATGGCA-3' & 5'-GGTAAATATCCTTTAATTAAA-3' with Biobricks prefix and suffix respectively.

Source of Origin Sequence

Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli Jeong-Woo Seo, Ki-Bang Song, Ki-Hyo Jang, Chul-Ho Kim, Bong-Hyun Jung, Sang-Ki Rhee * Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon 305 -600, South Korea

Pitfalls

Wild type K12 E coli contains LsrA

References

Molecular cloning of a gene encoding the thermoactive levansucrase from Rahnella aquatilis and its growth phase-dependent expression in Escherichia coli Jeong-Woo Seo, Ki-Bang Song, Ki-Hyo Jang, Chul-Ho Kim, Bong-Hyun Jung, Sang-Ki Rhee *Microbial and Bioprocess Engineering Laboratory, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon 305 -600, South Korea

Regulation of Uptake and Processing of the Quorum-Sensing Autoinducer AI-2 in Escherichia coli Karina B. Xavier and Bonnie L. Bassler* Department of Molecular Biology, Princeton University, Princeton, New Jersey