Composite

Part:BBa_K3467007:Design

Designed by: Wang Ran   Group: iGEM20_CAU_China   (2020-10-22)
Revision as of 18:11, 22 October 2020 by Registry (Talk | contribs)

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CarH-VP16 protein


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 673
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1270
    Illegal NgoMIV site found at 1276
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 168


Design Notes

This part has a promoter TEF, team who wants to use this part should first notice their plasmid first, checking whether their plasmid has the same promoter, in order to prevent the part from lost by homology recommbination.


Source

Chatelle, C. et al., A Green-Light-Responsive System for the Control of Transgene Expression in Mammalian and Plant Cells. ACS SYNTH BIOL 7 1349 (2018).

References