Composite

Part:BBa_K3510003

Designed by: team work   Group: iGEM20_Tuebingen   (2020-10-15)
Revision as of 17:06, 22 October 2020 by Lealinevogt (Talk | contribs)

Mn-Promoter-Mn-Riboswitch-FAST-Phytochelatine-Terminator

This composite part is a manganese inducible expression system of phytochelatine (BBa_K1321005) tagged with FAST2 (BBa_K3510000). Expression control consists of two individual elements: the manganese inducible promoter (BBa_K902073) and a manganese riboswitch (BBa_K902074). Transcription is regulated by the promoter, while the riboswitch supports translation initiation only in the presence of manganese. We combined both elements hoping for an even tighter regulation. Transcriptional termination occurs through the activity of the reliable double terminator (BBa_B0015).

Usage: In our project we coupled this manganese sensing device with a phytochelatin in order to create a bifunctional biosensor, that is not only able to detect manganese, but also directly binds it. With our suggested implementation this would contribute to the increase of water quality. Unlike the biosensor with the chromoprotein, the FAST2 tag allows a quantitative measurement of the fluorescence, which should provide information about the manganese concentration in the sample.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 327



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