Composite

Part:BBa_K3331011

Designed by: Wang Weizhe   Group: iGEM20_XJTU-China   (2020-10-22)
Revision as of 14:33, 22 October 2020 by Registry (Talk | contribs)

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B.S galU+P.A pgmA

This part can increase the yield of EPS. The yield of EPS can be significantly increased by overexpressing two key enzymes galU and pgmA at the same time. Reference: Levander Fredrik et al. Applied and environmental microbiology,2002,68(2). The part galU is from Bacillus subtilis subsp. subtilis str. 168. The part pgmA is from Pseudomonas aeruginosa PAO1.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1557
    Illegal BglII site found at 1590
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 912
    Illegal AgeI site found at 1146
    Illegal AgeI site found at 1785
    Illegal AgeI site found at 2163
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None