Reporter

Part:BBa_K3338001:Design

Designed by: Jonas Scholz   Group: iGEM20_Hannover   (2020-10-22)
Revision as of 13:47, 22 October 2020 by Jonas Scholz (Talk | contribs) (→‎References)

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Human codon optimized Gaussia luciferase hGLuc


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The sequence of the Gaussia Luciferase is codon optimized for expression in human cells.


Source

The sequence was purchased from Addgene (Plasmid #22522) (Pastrana et al. 2009).

References

Pastrana, D. V., Tolstov, Y. L., Becker, J. C., Moore, P. S., Chang, Y., & Buck, C. B. (2009). Quantitation of human seroresponsiveness to Merkel cell polyomavirus. PLoS pathogens, 5(9), e1000578.