Composite

Part:BBa_K3328021:Design

Designed by: Qi Wu   Group: iGEM20_OUC-China   (2020-10-04)
Revision as of 12:49, 22 October 2020 by Felix Felicis (Talk | contribs)

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switch of AND gate


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 753


Design Notes

AND gate constructed from two input RNAs that bind to yield a complete trigger RNA. When either input RNA is expressed, it is incapable of activating the switch because neither trigger sub-sequence alone can unwind the repressing hairpin. The toehold switch can only be turned on when the two input RNA species hybridize and form a complete trigger sequence.

799px-T--OUC-China--design_lunbo_and.jpg

Source

synthesize from company


References

Green, A., Kim, J., Ma, D. et al. Complex cellular logic computation using ribocomputing devices. Nature 548, 117–121 (2017). https://doi.org/10.1038/nature23271