Composite

Part:BBa_K3328026

Designed by: Qi Wu   Group: iGEM20_OUC-China   (2020-10-04)
Revision as of 12:43, 22 October 2020 by Felix Felicis (Talk | contribs)


triggers of NIMPLY gate (NIMPLY2)

two input trigger of NIMPLY2 logic gate to perform the truth table

Design

In the NIMPLY gate, a deactivating RNA (INPUT A) uses direct hybridization or strand displacement to abolish trigger RNA (input B) activity. So when only the correct trigger RNA is expressed, the switch can be turned on.

799px-T--OUC-China--design_lunbo_nimply.jpg

Result

800px-T--OUC-China--part_NIMPLY2.jpg

The left figure shows that when INPUT A=0, INPUT B=1, the fluorescence of GFP is high. And the fluorescence intensity of GFP was low in the other three groups. This corresponds to the situation described in the truth table on the right. INPUT A=1 means that aTc (0.25 mg/ml) is added, INPUT B=1 means that HSL (0.1 mg/ml) is added.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 110
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 107
    Illegal XhoI site found at 1
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 110
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 110
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
None