Composite
Part:BBa_K3606029:Design
Designed by: Gaochen Zhang Group: iGEM20_Fudan (2020-10-22)
P2 driven mcbABCD and PtetR driven mcbEFG
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal PstI site found at 2337
Illegal PstI site found at 2370 - 12INCOMPATIBLE WITH RFC[12]Illegal PstI site found at 2337
Illegal PstI site found at 2370 - 21COMPATIBLE WITH RFC[21]
- 23INCOMPATIBLE WITH RFC[23]Illegal PstI site found at 2337
Illegal PstI site found at 2370 - 25INCOMPATIBLE WITH RFC[25]Illegal PstI site found at 2337
Illegal PstI site found at 2370
Illegal NgoMIV site found at 1999
Illegal AgeI site found at 2171 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
McbABCD and McbEFG are placed in reverse, and each is independently expressed under different promoters with their own terminator.
Source
McbABCD and McbEFG orginally come from a plasmid of Escherichia coli.