Composite

Part:BBa_K3606029:Design

Designed by: Gaochen Zhang   Group: iGEM20_Fudan   (2020-10-22)
Revision as of 12:36, 22 October 2020 by Registry (Talk | contribs)

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P2 driven mcbABCD and PtetR driven mcbEFG


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2337
    Illegal PstI site found at 2370
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2337
    Illegal PstI site found at 2370
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2337
    Illegal PstI site found at 2370
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2337
    Illegal PstI site found at 2370
    Illegal NgoMIV site found at 1999
    Illegal AgeI site found at 2171
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

McbABCD and McbEFG are placed in reverse, and each is independently expressed under different promoters with their own terminator.


Source

McbABCD and McbEFG orginally come from a plasmid of Escherichia coli.

References