Composite

Part:BBa_K3606029

Designed by: Gaochen Zhang   Group: iGEM20_Fudan   (2020-10-22)
Revision as of 12:36, 22 October 2020 by Registry (Talk | contribs)

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P2 driven mcbABCD and PtetR driven mcbEFG

We used McbA-D to inhibit the growth of other bacteria in human intestine so as to enhance Nissle's competitiveness,as well as to reduce the risk of illness caused by some opportunistic pathogens. We introduced McbE-G to inhibit the growth of other bacteria in human intestine so as to enhance Nissle's competitiveness,as well as to reduce risk of illness caused by some opportunistic pathogens.At the same time, we put McbE-G under the control of pTetR in order to response to AHL-LuxR.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 2337
    Illegal PstI site found at 2370
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 2337
    Illegal PstI site found at 2370
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 2337
    Illegal PstI site found at 2370
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 2337
    Illegal PstI site found at 2370
    Illegal NgoMIV site found at 1999
    Illegal AgeI site found at 2171
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
//awards/composite_part/nominee
Parameters
None