Composite

Part:BBa_K3394005:Design

Designed by: Talia Shaler   Group: iGEM20_BGU-Israel   (2020-10-17)
Revision as of 12:06, 22 October 2020 by Talia sh (Talk | contribs) (→‎Source)


Cellulolytic complex [2]: His-CBD-GS-Endo5a


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1623
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1623
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1623
    Illegal BamHI site found at 28
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1623
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 76
    Illegal EcoRI site found at 1623
    Illegal AgeI site found at 51
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We made another design in which the gene order is different: first the cellulase endo5a and then the CBD.


Source

References

Adlakha, N., Rajagopal, R., Kumar, S., Reddy, V. S., & Yazdani, S. S. (2011). Synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect gut bacterium. Applied and environmental microbiology, 77(14), 4859-4866.

Gupta, S., Adlakha, N., & Yazdani, S. S. (2013). Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli. Protein expression and purification, 88(1), 20-25.