Composite
Part:BBa_K3394004:Design
Designed by: Talia Shaler Group: iGEM20_BGU-Israel (2020-10-11)
Cellulolytic complex [1]: His-Endo5a-GS-CBD
Assembly Compatibility:
- 10INCOMPATIBLE WITH RFC[10]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314 - 12INCOMPATIBLE WITH RFC[12]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314 - 21INCOMPATIBLE WITH RFC[21]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314
Illegal BamHI site found at 28 - 23INCOMPATIBLE WITH RFC[23]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314 - 25INCOMPATIBLE WITH RFC[25]Illegal EcoRI site found at 988
Illegal EcoRI site found at 1314
Illegal AgeI site found at 1289 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
Source
References
Adlakha, N., Rajagopal, R., Kumar, S., Reddy, V. S., & Yazdani, S. S. (2011). Synthesis and characterization of chimeric proteins based on cellulase and xylanase from an insect gut bacterium. Applied and environmental microbiology, 77(14), 4859-4866.
Gupta, S., Adlakha, N., & Yazdani, S. S. (2013). Efficient extracellular secretion of an endoglucanase and a β-glucosidase in E. coli. Protein expression and purification, 88(1), 20-25.