Device
Part:BBa_K3402055
Designed by: Yibo Shi Group: iGEM20_Jiangnan_China (2020-10-22)
Over-expression UGTB cassette
This device is composed of 50bp-upPXA1(BBa_K3402037), Six site(BBa_K3402032), Pgalk(BBa_K3402033), Rec(BBa_K3402034), Tgki(BBa_K3402019), hph(BBa_K3402012), Ptef1(BBa_K3402007), UGTB(BBa_K3402011), Tsyn7(BBa_K3402001), 50bp-doPXA1(BBa_K3402038).
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 1076
Illegal BglII site found at 5893
Illegal BamHI site found at 3479
Illegal XhoI site found at 1910
Illegal XhoI site found at 4631 - 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 219
Illegal AgeI site found at 5315 - 1000INCOMPATIBLE WITH RFC[1000]Illegal BsaI site found at 5474
Illegal BsaI.rc site found at 5429
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Categories
Parameters
None |