Part:BBa_K3447007

xynD, which produces xylanase

P_BAD promoter is an inducible promoter, P_BAD contains sequences of PC. It was verified by iGEM 2017 Amazonas_Brazil team that the induction effect of arabinose could be realized by using only araC and P_BAD.

Usage and Biology

In the arabinose operon, it is regulated by the AraC operon and activates the expression of downstream arabinose metabolism genes in the presence of arabinose inducers.
The L-Arabinose operon is naturally present in E. coli.The regulatory protein AraC ACTS as a dimer. The operon contains two cis-type control elements: The P_C promoter for the synthesis of AraC and the P_BAD for the synthesis of the enzyme needed for the catabolism of L-Arabinose. P_BAD contains three half sites, each of which binds to a subunit of ARAC-O2, I1, and I2. The O1 site is composed of O1L and O1R half sites that bind two subunits. Half of O2 is in the araC coding region. We connected the target genes downstream of P_BAD to constitute the effect part of arabinose sensing system, and constructed the whole arabinose sensing system on the same plasmid with P_C and araC. Arabinose induces endotoxin expression: BBa_K3447000 Arabinose induces green fluorescent protein expression: (链接a01+c09)

Sequence and Features

Assembly Compatibility:

• 10
  INCOMPATIBLE WITH RFC[10]

  Illegal PstI site found at 216
  Illegal PstI site found at 226
  
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal PstI site found at 216
  Illegal PstI site found at 226
  
• 21
  COMPATIBLE WITH RFC[21]
• 23
  INCOMPATIBLE WITH RFC[23]

  Illegal PstI site found at 216
  Illegal PstI site found at 226
  
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal PstI site found at 216
  Illegal PstI site found at 226
  
• 1000
  COMPATIBLE WITH RFC[1000]