Coding
Part:BBa_K3335004
Designed by: TianYu Ma Group: iGEM20_NJU-China (2020-10-22)
We used it to express iRGD-Lamp2b and siRNA targeted at PD-L1
Tumor cells evade immune system surveillance by overexpressing PD-L1.Therefore, we combined the fusion expression of iRGD and Lamp2b to characterize the exosome targeting.At the same time, siRNA targeting PD-L1 is produced to reduce mRNA expression.This allows the immune system to avoid the effects of PD-L1 and attack tumor cells.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 26
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
[edit]
Categories
Parameters
None |